To preserve the cultural resources of the Route 66 corridor and to authorize the Secretary of the Interior to provide assistance.

Directs the Secretary of the Interior to: (1) facilitate the development of guidelines and a program of technical assistance and grants that will set priorities for the preservation of the Route 66 corridor; (2) designate National Park Service (NPS) officials to perform the functions of the Cultural Resource Programs support cultural resources related to the corridor; (3) support efforts of State and local public and private persons, nonprofit Route 66 preservation entities, Indian tribes, State Historic Preservation Offices, and entities in the States to preserve the corridor by providing technical assistance, participating in cost-sharing programs, and making grants; (4) act as a clearinghouse for communication among such parties and Federal, State, and local agencies; and (5) assist the States in determining the appropriate form of, and establishing and supporting, a non-Federal entity or entities to perform the functions of the Cultural Resource Programs after those programs are terminated.

Authorizes the Secretary to: (1) enter into cooperative agreements; (2) accept donations of funds, equipment, supplies, and services as appropriate; (3) provide cost-share grants for up to 50 percent of projects for the preservation of the corridor and information about existing cost-share opportunities and technical assistance in the corridor's historic preservation and interpretation; and (4) coordinate, promote, and stimulate research by other persons and entities regarding the corridor.

Requires the Secretary: (1) to provide assistance in the preservation of the corridor that is compatible with the idiosyncratic nature of the highway; (2) not to prepare or require an overall management plan, but to cooperate with public and private entities in developing local preservation plans to guide efforts to protect the most important or representative resources of the corridor; (3) to develop a technical assistance program in the preservation and interpretation of the corridor, including guidelines for setting priorities for preservation needs; and (4) to coordinate a program of historic research, curation, preservation strategies, and collection of oral and video histories of events that occurred along the corridor designed for continuing use after the Cultural Resource Programs are terminated.

Authorizes appropriation.